Hu, P., Wu, S., Hernandez, N.
(September 2003)
A minimal RNA polymerase III transcription system from human cells reveals positive and negative regulatory roles for CK2.
Molecular Cell, 12 (3).
pp. 699-709.
ISSN 1097-2765
Abstract
In higher eukaryotes, RNA polymerase (pol) III is known to use different transcription factors to recognize three basic types of promoters, but in no case have these transcription factors been completely defined. We show that a highly purified pol III complex combined with the recombinant transcription factors SNAP(c), TBP, Brf2, and Bdp1 directs multiple rounds of transcription initiation and termination from the human U6 promoter. The pol III complex contains traces of CK2, and CK2 associates with the U6 promoter region in vivo. Transcription requires CK2 phosphorylation of the pol III complex. In contrast, CK2 phosphorylation of TBP, Brf2, and Bdp1 combined is inhibitory. The results define a minimum core machinery, the ultimate target of regulatory mechanisms, capable of directing all steps of the transcription process-initiation, elongation, and termination-by a metazoan RNA polymerase, and suggest positive and negative regulatory roles for CK2 in transcription by pol III.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
CASEIN KINASE-II
TATA-BINDING PROTEIN
ACCURATE TERMINATION
INITIATION-FACTOR
STABLE COMPLEX
YEAST TFIIIB
IN-VITRO
PROMOTER
GENES
COMPONENT |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hernandez lab |
| Depositing User: |
Matt Covey
|
| Date: |
September 2003 |
| Date Deposited: |
02 Apr 2013 13:49 |
| Last Modified: |
02 Apr 2013 13:49 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27990 |
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