Stenlund, A., Zabielski, J., Ahola, H., Moreno-Lopez, J., Pettersson, U. (April 1985) Messenger RNAs from the transforming region of bovine papilloma virus type I. J Mol Biol, 182 (4). pp. 541-54. ISSN 0022-2836 (Print)0022-2836 (Linking)
Abstract
Messenger RNAs present in C127 mouse cells transformed by bovine papilloma virus type 1 (BPV-1) were studied by the S1 nuclease protection technique, Northern blotting, and electron microscopic heteroduplex analysis. The results revealed at least five classes of spliced mRNAs which we designate types 1 to 5. They had a common poly(A) addition site located at co-ordinate 53 and all mRNAs, except the type 3 mRNAs, contained an exon located between co-ordinates 41 and 53. In the type 1 mRNAs this exon was connected to a very short leader sequence located around co-ordinate 31. The type 2 mRNAs contained 220 to 400-nucleotide long leaders which were located approximately 1.5 X 10(3) base-pairs further upstream. Two different subclasses of type 2 molecules (2A and 2B) were identified and these had slightly different leaders. The type 4 mRNAs contained a bipartite leader, whereas the type 5 mRNAs carried an approximately 900-nucleotide long leader. The type 3 mRNAs consisted of a main exon located between co-ordinates 32 and 53, linked to the same leader as is present in the type 2A mRNAs. A cap site which presumably is utilized by the type 2A, type 3, type 4 and type 5 mRNAs was mapped at nucleotide 89 in the BPV-1 sequence. A putative cap site for the type 1 mRNAs was mapped at co-ordinate 31.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Bovine papillomavirus 1/ genetics Cattle Cell Transformation, Viral Cells, Cultured DNA, Viral Endonucleases Genes, Viral Mice Microscopy, Electron Nucleic Acid Hybridization Papillomaviridae/ genetics RNA, Heterogeneous Nuclear/analysis RNA, Messenger/analysis/ genetics RNA, Viral/analysis/ genetics Single-Strand Specific DNA and RNA Endonucleases |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Matt Covey |
| Date: | 20 April 1985 |
| Date Deposited: | 12 Mar 2013 18:58 |
| Last Modified: | 12 Mar 2013 18:58 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27776 |
Actions (login required)
 |
Administrator's edit/view item |
