Synaptic vesicle recycling studied in transgenic mice expressing synaptopHluorin

Li, Z., Burrone, J., Tyler, W. J., Hartman, K. N., Albeanu, D. F., Murthy, V. N. (2005) Synaptic vesicle recycling studied in transgenic mice expressing synaptopHluorin. Proceedings of the National Academy of Sciences of the United States of America, 102 (17). pp. 6131-6136. ISSN 00278424 (ISSN)

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DOI: 10.1073/pnas.0501145102


Synaptic vesicles are recycled locally within presynaptic specializations. We examined how vesicles are reused after endocytosis, using transgenic mice expressing the genetically encoded fluorescent indicator synaptopHluorin in subsets of neurons. At both excitatory and inhibitory synapses in cultured hippocampal neurons, newly endocytosed vesicles did not preferentially enter the releasable pool of vesicles. Rather, they entered the reserve pool first and subsequently the readily releasable pool over a period of several minutes. All vesicles in the recycling pool could be accessed by spaced stimuli, arguing against preferential local reuse of the readily releasable vesicles. Interestingly, nearly half the vesicles at excitatory synapses, and a third at inhibitory synapses, could not be recruited for release even by sustained stimuli. We conclude that, at presynaptic terminals in the hippocampus, most vesicles vacate release sites after exocytosis and are replaced by existing vesicles from the reserve pool, placing constraints on kiss-and-run recycling. © 2005 by The National Academy of Sciences of the USA.

Item Type: Paper
Uncontrolled Keywords: Endocytosis Exocytosis Hippocampus Kiss and run membrane protein synaptophluorin unclassified drug animal cell article mouse nerve cell culture nerve ending newborn nonhuman priority journal protein expression synapse synapse vesicle transgenic mouse Animals Animals, Newborn Cells, Cultured Green Fluorescent Proteins Mice Mice, Transgenic Nerve Tissue Proteins Neurons Patch-Clamp Techniques Synapses Synaptic Vesicles Mus musculus
Subjects: organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Albeanu lab
Depositing User: CSHL Librarian
Date: 2005
Date Deposited: 19 Mar 2012 20:59
Last Modified: 11 Jan 2018 20:18
PMCID: PMC1087931
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