Coupling of Neural Activity to Blood Flow in Olfactory Glomeruli Is Mediated by Astrocytic Pathways

Petzold, G. C., Albeanu, D. F., Sato, T. F., Murthy, V. N. (2008) Coupling of Neural Activity to Blood Flow in Olfactory Glomeruli Is Mediated by Astrocytic Pathways. Neuron, 58 (6). pp. 897-910. ISSN 08966273 (ISSN)

DOI: 10.1016/j.neuron.2008.04.029


Functional neuroimaging uses activity-dependent changes in cerebral blood flow to map brain activity, but the contributions of presynaptic and postsynaptic activity are incompletely understood, as are the underlying cellular pathways. Using intravital multiphoton microscopy, we measured presynaptic activity, postsynaptic neuronal and astrocytic calcium responses, and erythrocyte velocity and flux in olfactory glomeruli during odor stimulation in mice. Odor-evoked functional hyperemia in glomerular capillaries was highly correlated with glutamate release, but did not require local postsynaptic activity. Odor stimulation induced calcium transients in astrocyte endfeet and an associated dilation of upstream arterioles. Calcium elevations in astrocytes and functional hyperemia depended on astrocytic metabotropic glutamate receptor 5 and cyclooxygenase activation. Astrocytic glutamate transporters also contributed to functional hyperemia through mechanisms independent of calcium rises and cyclooxygenase activation. These local pathways initiated by glutamate account for a large part of the coupling between synaptic activity and functional hyperemia in the olfactory bulb. © 2008 Elsevier Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: MOLNEURO Molneuro SYSNEURO Sysneuro glutamate receptor 5 glutamate transporter prostaglandin synthase article astrocyte astrocytosis brain blood flow cell activity controlled study electric activity electroencephalogram enzyme activation erythrocyte fluorescence glomerulus capillary hyperemia mouse multiphoton microscopy nerve cell neurotransmitter release nonhuman olfactory bulb postsynaptic inhibition priority journal smelling synaptic transmission Animals Astrocytes Cerebrovascular Circulation Mice Olfactory Receptor Neurons Receptors Odorant Signal Transduction
Subjects: Investigative techniques and equipment
organs, tissues, organelles, cell types and functions > organs types and functions > brain
Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Albeanu lab
Depositing User: CSHL Librarian
Date: 2008
Date Deposited: 19 Mar 2012 20:51
Last Modified: 04 Dec 2014 16:44
PMCID: PMC2922004
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