Dalma-Weiszhausz, D. D., Chicurel, M. E., Gingeras, T. R. (2002) Microarrays and genetic epidemiology: A multipurpose tool for a multifaceted field. Genetic Epidemiology, 23 (1). pp. 4-20. ISSN 07410395 (ISSN)
Abstract
The advent of molecular technologies that allow the collection and analysis of large amounts of genetic data is rapidly transforming the field of genetic epidemiology. Whether monitoring infectious outbreaks or identifying genotypic variations that underlie disease susceptibility, genetic epidemiology relies heavily on the analysis of multiple, independently derived results. By allowing the simultaneous monitoring of thousands of genetic or expression data points, microarrays are emerging as particularly powerful tools. Several recent reviews have described array manufacturing and the types of scientific questions that can exploit this technology, but few have addressed how the intended use of an array can dictate its design. This review will focus on this latter issue, with particular emphasis on the genetic epidemiology of infectious disease. The design of arrays for genotyping, expression profiling, and fingerprinting are presented, and examples of recent epidemiological studies are used to illustrate the applications' strong points and limitations. In addition to discussing arrays' ability to provide global views of gene identity or function, the review will describe design options for creating arrays that detect multiple genetic variations. It will also examine the reliability of array-generated fingerprints, assay accessibility, and possibilities for sharing and comparing data across studies. Although many challenges lie ahead, microarrays' multiple abilities appear uniquely poised to accelerate the advance of genetic epidemiology's multiple fronts. © 2002 Wiley-Liss, Inc.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA arrays Expression profiling Fingerprinting Genetic epidemiology Genomics Genotyping Infectious disease Oligonucleotide microarrays Single-nucleotide polymorphism DNA oligonucleotide article DNA fingerprinting DNA microarray gene expression genetic variability genotype human infection single nucleotide polymorphism chemistry DNA probe epidemiology genetics methodology nucleotide sequence reproducibility review Base Sequence DNA Probes Epidemiologic Methods Oligonucleotide Array Sequence Analysis Reproducibility of Results Research Design Variation Genetics Humans |
| Subjects: | bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 2002 |
| Date Deposited: | 13 Mar 2012 13:59 |
| Last Modified: | 15 Jul 2013 15:53 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25285 |
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