A computer assisted method for the determination of restriction enzyme recognition sites

Gingeras, T. R., Milazzo, J. P., Roberts, R. J. (1978) A computer assisted method for the determination of restriction enzyme recognition sites. Nucleic Acids Research, 5 (11). pp. 4105-4128. ISSN 03051048 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/724510
DOI: 10.1093/nar/5.11.4105


A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of øX174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method. © 1978 Information Retrieval Limited 1 Falconberg Court London W1V 5FG England.

Item Type: Paper
Uncontrolled Keywords: DNA oligodeoxyribonucleotide restriction endonuclease article computer nucleotide sequence technique Base Sequence Computers DNA Restriction Enzymes Methods Oligodeoxyribonucleotides
Subjects: bioinformatics > genomics and proteomics > computers > computer software
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
CSHL Authors:
Communities: CSHL labs > Gingeras lab
CSHL labs > Roberts lab
Depositing User: CSHL Librarian
Date: 1978
Date Deposited: 14 Mar 2012 15:20
Last Modified: 31 Mar 2014 14:54
PMCID: PMC342737
Related URLs:
URI: https://repository.cshl.edu/id/eprint/25220

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