Gaillard, P. H., Martini, E. M., Kaufman, P. D., Stillman, B., Moustacchi, E., Almouzni, G. (September 1996) Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I. Cell, 86 (6). pp. 887-96. ISSN 0092-8674 (Print)
Abstract
DNA repair in the eukaryotic cell disrupts local chromatin organization. To investigate whether the resetting of nucleosomal arrays can be linked to the repair process, we developed model systems, with both Xenopus egg extract and human cell extracts, to follow repair and chromatin assembly in parallel on circular DNA templates. Both systems were able to carry out nucleotide excision repair of DNA lesions. We observed that UV-dependent DNA synthesis occurs simultaneously with chromatin assembly, strongly indicating a mechanistic coupling between the two processes. A complementation assay established that chromatin assembly factor I (CAF1) is necessary for this repair associated chromatin formation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Cell Line Chromatin metabolism Chromosomal Proteins Non-Histone DNA Repair physiology DNA-Binding Proteins metabolism Female Humans Models Biological Oocytes metabolism Plasmids metabolism radiation effects Recombinant Proteins metabolism Ultraviolet Rays Xenopus |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nucleosome |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 20 September 1996 |
| Date Deposited: | 06 Mar 2012 17:04 |
| Last Modified: | 20 Jun 2017 19:37 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24980 |
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