Komarova, A. V., Rea, E., Borman, A. M., Brocard, M., England, P., Tordo, N., Hershey, J. W. B., Kean, K. M., Jacob, Y. (March 2007) Rabies virus matrix protein interplay with elF3, new insights into rabies virus pathogenesis. Nucleic Acids Research, 35 (5). pp. 1522-1532. ISSN 03051048 (ISSN)
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Abstract
Viral proteins are frequently multifunctional to accommodate the high density of information encoded in viral genomes. Matrix (M) protein of negative-stranded RNA viruses such as Rhabdoviridae is one such example. Its primary function is virus assembly/budding but it is also involved in the switch from viral transcription to replication and the concomitant down regulation of host gene expression. In this study we undertook a search for potential rabies virus (RV) M protein's cellular partners. In a yeast two-hybrid screen the elF3h subunit was identified as an M-interacting cellular factor, and the interaction was validated by co-immunoprecipitation and surface plasmon resonance assays. Upon expression in mammalian cell cultures, RV M protein was localized in early small ribosomal subunit fractions. Further, M protein added in trans inhibited in vitro translation on mRNA encompassing classical (Kozak-like) 5'-UTRs. Interestingly, translation of hepatitis C virus IRES-containing mRNA, which recruits elF3 via a different noncanonical mechanism, was unaffected. Together, the data suggest that, as a complement to its functions in virus assembly/budding and regulation of viral transcription, RV M protein plays a role in inhibiting translation in virus-infected cells through a proteinprotein interaction with the cellular translation machinery.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | VESICULAR STOMATITIS-VIRUS TRANSLATION INITIATION COMPLEX BINDING-PROTEIN EUKARYOTIC INITIATION-FACTOR-3 CAP-POLY(A) SYNERGY MESSENGER-RNAS GLYCOPROTEIN G ENTRY SITES HEPATITIS-C UBIQUITIN |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > matrix protein organism description > virus > rabies virus organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL Post Doctoral Fellows CSHL labs > Malinow lab |
| Depositing User: | CSHL Librarian |
| Date: | March 2007 |
| Date Deposited: | 11 Nov 2011 19:05 |
| Last Modified: | 02 May 2013 16:52 |
| PMCID: | PMC1865048 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/23069 |
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