Di Cristo, C., Fiore, G., Scheinker, V., Enikolopov, G. N., d'Ischia, M., Palumbo, A., Di Cosmo, A. (September 2007) Nitric oxide synthase expression in the central nervous system of Sepia officinalis: an in situ hybridization study. European Journal of Neuroscience, 26 (6). pp. 1599-1610. ISSN 0953-816X
Abstract
We recently reported the molecular cloning of nitric oxide synthase (NOS) mRNA from Sepia officinalis (SoNOS) using a strategy that involves hybridization of degenerate PCR primers to highly conserved NOS regions, combined with a RACE procedure. Here, in situ hybridization study has been performed on serial sections of the cuttlefish central nervous system to reveal localized specific staining of cell bodies in several lobes of the brain. Staining was found in many lower motor centres, including cells of the inferior and superior buccal lobes (feeding centres); in some higher motor centres (anterior basal and peduncle lobes); in learning centres (vertical, subvertical and superior frontal lobes); and in the visual system [medulla and deep retina (optic lobe)]. Positive staining was also found in the olfactory lobe. NOS-expressing cells have been detected also in the interbasal lobe. Double labelling experiments, performed on consecutive sections, showed that neurons containing NOS immunoreactivity were also positive in in situ hybridization staining. All these data support the presence of NOS in several systems in the cuttlefish brain.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > animal > fish > cuttlefish bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > nitric oxide synthase |
| CSHL Authors: | |
| Communities: | CSHL labs > Enikopolov lab |
| Depositing User: | CSHL Librarian |
| Date: | September 2007 |
| Date Deposited: | 30 Nov 2011 20:30 |
| Last Modified: | 28 Feb 2017 22:03 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22999 |
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