Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages

Seaberg, R. M., Smukler, S. R., Kieffer, T. J., Enikolopov, G., Asghar, Z., Wheeler, M. B., Korbutt, G., van der Kooy, D. (September 2004) Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages. Nature Biotechnology, 22 (9). pp. 1115-1124. ISSN 1087-0156

DOI: 10.1038/nbt1004


The clonal isolation of putative adult pancreatic precursors has been an elusive goal of researchers seeking to develop cell replacement strategies for diabetes. We report the clonal identification of multipotent precursor cells from the adult mouse pancreas. The application of a serum-free, colony-forming assay to pancreatic cells enabled the identification of precursors from pancreatic islet and ductal populations. These cells proliferate in vitro to form clonal colonies that coexpress neural and pancreatic precursor markers. Upon differentiation, individual clonal colonies produce distinct populations of neurons and glial cells, pancreatic endocrine beta-, alpha- and delta-cells, and pancreatic exocrine and stellate cells. Moreover, the newly generated b- like cells demonstrate glucose-dependent Ca2+ responsiveness and insulin release. Pancreas colonies do not express markers of embryonic stem cells, nor genes suggestive of mesodermal or neural crest origins. These cells represent a previously unidentified adult intrinsic pancreatic precursor population and are a promising candidate for cell-based therapeutic strategies.

Item Type: Paper
Uncontrolled Keywords: HEMATOPOIETIC STEM-CELLS hematopoietic stem cells NESTIN-POSITIVE PRECURSORS nestin posative procursors PROGENITOR CELLS progenitor cells BETA-CELLS Beta-Cells Beta cells TRANSCRIPTION FACTOR transcription factor ENDOTHELIAL-CELLS endothelial cells EXPRESSING CELLS expressing cells NERVOUS-SYSTEM nervous system EX-VIVO ex-vivo MICE mice
Subjects: diseases & disorders > nutritional and metabolic diseases > diabetes
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: CSHL Librarian
Date: September 2004
Date Deposited: 26 Jan 2012 14:31
Last Modified: 01 Mar 2017 16:54
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