Cholinergic-mediated IP3-ereceptor activation induces long-lasting synaptic enhancement in CA1 pyramidal neurons

de Sevilla, D. F., Núñez, A., Borde, M., Malinow, R., Buño, W. (2008) Cholinergic-mediated IP3-ereceptor activation induces long-lasting synaptic enhancement in CA1 pyramidal neurons. Journal of Neuroscience, 28 (6). pp. 1469-1478. ISSN 0270-6474

[img]
Preview
PDF
Cholinergic_mediated.pdf - Published Version

Download (851Kb)
URL: http://www.jneurosci.org/cgi/content/full/28/6/146...

Abstract

Cholinergic–glutamatergic interactions influence forms of synaptic plasticity that are thought to mediate memory and learning. We tested in vitro the induction of long-lasting synaptic enhancement at Schaffer collaterals by acetylcholine (ACh) at the apical dendrite of CA1 pyramidal neurons and in vivo by stimulation of cholinergic afferents. In vitro ACh induced a Ca2+ wave and synaptic enhancement mediated by insertion of AMPA receptors in spines. Activation of muscarinic ACh receptors (mAChRs) and Ca2+ release from inositol 1,4,5-trisphosphate (IP3)-sensitive stores were required for this synaptic enhancement that was insensitive to blockade of NMDA receptors and also triggered by IP3 uncaging. Activation of cholinergic afferents in vivo induced an analogous atropine-sensitive synaptic enhancement. We describe a novel form of synaptic enhancement (LTPIP3) that is induced in vitro and in vivo by activation of mAChRs. We conclude that Ca2+ released from postsynaptic endoplasmic reticulum stores is the critical event in the induction of this unique form of long-lasting synaptic enhancement.

Item Type: Paper
Additional Information: DOI 10.1523/JNEUROSCI.2723-07.2008
Uncontrolled Keywords: LTPIP3 endoplasmic reticulum Ca2+ wave AMPA trafficking inositol MUSCARINIC RECEPTORS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Depositing User: Tom Adams
Date Deposited: 25 Aug 2011 17:55
Last Modified: 29 Nov 2011 20:09
URI: http://repository.cshl.edu/id/eprint/7715

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving