Qu, S. X., Wang, X. F., Li, H. P., Luo, X., Ma, L. (July 2018) A Gustatory Receptor Used for Rapid Detection of Tyrophagus putrescentiae in Fungi Hosts. Sci Rep, 8 (1). p. 11425. ISSN 2045-2322
Abstract
The storage mite, Tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in China. Storage mites are tiny and difficult to observe, especially when they occur in fungi composts. In this study, one gustatory receptor protein (TputGR1) was identified from the transcriptome of T. putrescentiae. Phylogenetic analysis of GRs families from 10 arthropod species revealed that TputGR1 had high homology with the SccaGR1 of Sarcoptes scabiei and TurtGR1-2 of Tetranychus urticae, but low homology with other insect species, Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Aedes aegypti, Culex quinquefasciatus, and Pediculus humanus. We developed a detection system for the mite on fungi hosts using the GR protein and the loop-mediated isothermal amplification (LAMP). This procedure was rapid (60 min from sampling to result) and had high sensitivity (0.5 ng/mL). LAMP provided rapid and reliable detection of T. putrescentiae. It has good specificity for single samples and for large-scale surveys.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor |
| CSHL Authors: | |
| Depositing User: | Matthew Dunn |
| Date: | 30 July 2018 |
| Date Deposited: | 14 Aug 2018 15:03 |
| Last Modified: | 14 Aug 2018 15:03 |
| PMCID: | PMC6065420 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/37128 |
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