Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters

Dergai, O., Cousin, P., Gouge, J., Satia, K., Praz, V., Kuhlman, T., Lhote, P., Vannini, A., Hernandez, N. (May 2018) Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. Genes Dev, 32 (9-10). pp. 711-722. ISSN 0890-9369

URL: https://www.ncbi.nlm.nih.gov/pubmed/29785964
DOI: 10.1101/gad.314245.118

Abstract

RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

Item Type: Paper
Uncontrolled Keywords: Brf2 SNAPc Tbp Tfiia Tfiib small nuclear RNA promoters
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Matt Covey
Date: 1 May 2018
Date Deposited: 25 May 2018 20:53
Last Modified: 14 Jun 2018 21:03
Related URLs:
URI: http://repository.cshl.edu/id/eprint/36595

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