The effect of hemolytic substances on white cell respiration

Ponder, Eric, MacLeod, John (1936) The effect of hemolytic substances on white cell respiration. J Gen Physiol., 20 (2). pp. 267-281.

URL: https://www.ncbi.nlm.nih.gov/pubmed/19872992

Abstract

Substances such as saponin, the bile salts, etc., which produce lysis of red cells also produce cytolysis of white cells from rabbit peritoneal exudates, the arbitrary criterion of their cytolytic effect being their ability to depress the O(2) consumption of the leucocytes. The amount of cytolysis increases regularly as the amount of the added lysin is increased, and sufficiently large quantities of saponin, sodium taurocholate, sodium glycocholate, or sodium oleate are capable of virtually abolishing the O(2) consumption altogether. At the same time, it can be shown that a lysin such as saponin is used up in combining with the white cells in much the same way as it is used up in combining with red cells, and the reduction in oxygen consumption appears to be roughly proportional to the amount so combined. The action of these lytic substances on white cells, in fact, is very similar to their action on red cells, due allowance being made for the fact that the cytolysis of the white cell is probably not an all-or-none process like hemolysis. White cell respiration is also depressed in hypotonic solutions, the respiration being virtually linear with the tonicity.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
Communities: The Biological Laboratory
Depositing User: Elizabeth Pessala
Date Deposited: 28 Sep 2017 14:54
Last Modified: 28 Sep 2017 14:54
PMCID: PMC2213725
URI: http://repository.cshl.edu/id/eprint/35532

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