Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity

Paul, A., Crow, M., Raudales, R., He, M., Gillis, J., Huang, Z. J. (2017) Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity. Cell.

URL: https://www.ncbi.nlm.nih.gov/pubmed/28942923
DOI: 10.1016/j.cell.2017.08.032

Abstract

Understanding the organizational logic of neural circuits requires deciphering the biological basis of neuronal diversity and identity, but there is no consensus on how neuron types should be defined. We analyzed single-cell transcriptomes of a set of anatomically and physiologically characterized cortical GABAergic neurons and conducted a computational genomic screen for transcriptional profiles that distinguish them from one another. We discovered that cardinal GABAergic neuron types are delineated by a transcriptional architecture that encodes their synaptic communication patterns. This architecture comprises 6 categories of ∼40 gene families, including cell-adhesion molecules, transmitter-modulator receptors, ion channels, signaling proteins, neuropeptides and vesicular release components, and transcription factors. Combinatorial expression of select members across families shapes a multi-layered molecular scaffold along the cell membrane that may customize synaptic connectivity patterns and input-output signaling properties. This molecular genetic framework of neuronal identity integrates cell phenotypes along multiple axes and provides a foundation for discovering and classifying neuron types.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > small molecules > GABAergic
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Gillis Lab
CSHL labs > Huang lab
Depositing User: Matt Covey
Date Deposited: 23 Aug 2017 15:05
Last Modified: 26 Sep 2017 20:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35251

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