Generation of Murine Stromal Cell-Lines Supporting Hematopoietic Stem-Cell Proliferation by Use of Recombinant Retrovirus Vectors Encoding Simian Virus-40 Large T-Antigen

Williams, D. A., Rosenblatt, M. F., Beier, D. R., Cone, R. D. (September 1988) Generation of Murine Stromal Cell-Lines Supporting Hematopoietic Stem-Cell Proliferation by Use of Recombinant Retrovirus Vectors Encoding Simian Virus-40 Large T-Antigen. Molecular and Cellular Biology, 8 (9). pp. 3864-3871. ISSN 0270-7306

URL: http://www.ncbi.nlm.nih.gov/pubmed/2851729
DOI: 10.1128/MCB.8.9.3864

Abstract

The bone marrow is a complex microenvironment made up of multiple cell types which appears to play an important role in the maintenance of hematopoietic stem cell self-renewal and proliferation. We used murine long-term marrow cultures and a defective recombinant retrovirus vector containing the simian virus 40 large T antigen to immortalize marrow stromal cells which can support hematopoiesis in vitro for up to 5 weeks. Such cloned cell lines differentially supported stem cells which, when transplanted, allowed survival of lethally irradiated mice, formed hematopoietic spleen colonies in vivo, and stimulated lymphocyte proliferation in vitro. Molecular and functional analyses of these cell lines did not demonstrate the production of any growth factors known to support the proliferation of primitive hematopoietic stem cells. All cell lines examined produced macrophage colony-stimulating factor. The use of immortalizing retrovirus vectors may allow determination of unique cellular proteins important in hematopoietic stem cell proliferation by the systematic comparison of stromal cells derived from a variety of murine tissues.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line

organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell

organism description > virus > retrovirus
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: September 1988
Date Deposited: 05 Sep 2017 16:01
Last Modified: 05 Sep 2017 16:01
PMCID: PMC365445
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35229

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