A new restriction endonuclease from Streptomyces albus G

Arrand, J. R., Myers, P. A., Roberts, R. J. (January 1978) A new restriction endonuclease from Streptomyces albus G. J Mol Biol, 118 (1). pp. 127-35. ISSN 0022-2836 (Print)0022-2836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/415144
DOI: 10.1016/0022-2836(78)90249-8

Abstract

A restriction endonuclease, SalI, has been partially purified from Streptomyces albus G. This enzyme cleaves adenovirus-2 DNA at three sites, bacteriophage λ DNA at two sites, but does not cleave simian virus 40 DNA or φX174 DNA. It recognizes the sequence and cuts at the sites indicated by the arrows. An endonuclease (XamI) with similar specificity has also been isolated from Xanthomonas amaranthicola.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Chromatography, DEAE-Cellulose DNA Restriction Enzymes/*isolation & purification/metabolism DNA, Viral/metabolism Electrophoresis, Agar Gel Streptomyces griseus/enzymology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
CSHL Authors:
Communities: CSHL labs > Roberts lab
Depositing User: Matt Covey
Date: 5 January 1978
Date Deposited: 25 Aug 2016 14:41
Last Modified: 25 Aug 2016 14:41
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33328

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