Nucleotide sequences of the attachment sites of bacteriophage Mu DNA

Kahmann, R., Kamp, D. (July 1979) Nucleotide sequences of the attachment sites of bacteriophage Mu DNA. Nature, 280 (5719). pp. 247-50. ISSN 0028-0836 (Print)0028-0836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/377106
DOI: 10.1038/280247a0

Abstract

THE temperate bacteriophage Mu has the remarkable ability to insert its DNA in apparently random sites of the Escherichia coli chromosome (1–3). All Mu prophages have the same gene order4, and the finding that the prophage and the phage maps are identical suggests that specific sites (attachment sites) at the ends of the Mu genome are used for the integration of Mu5. Phage particles do not contain the excised, free form of the Mu DNA; instead, the Mu genome is flanked by what seems to be heterogeneous bacterial DNA6,7. The variable DNA at both ends of Mu is thought to be generated when Mu DNA is transposed to many new chromosomal sites during replication and is subsequently packaged into phage particles together with adjacent host DNA. The nucleotide sequence analysis of the ends of Mu DNA reported here substantiates this hypothesis and identifies the attachment sites of Mu. Structural features of the attachment sites are presented.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Coliphages/*genetics DNA, Viral/genetics Escherichia coli/*genetics Receptors, Virus/*analysis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage

organism description > bacteria > escherichia coli
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 19 July 1979
Date Deposited: 25 May 2016 19:33
Last Modified: 25 May 2016 19:33
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32694

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