The Adenovirus-E1a 12s Product Displays Functional Redundancy in Activating the Human Proliferating Cell Nuclear Antigen Promoter

Kannabiran, C., Morris, G. F., Labrie, C., Mathews, M. B. (January 1993) The Adenovirus-E1a 12s Product Displays Functional Redundancy in Activating the Human Proliferating Cell Nuclear Antigen Promoter. Journal of Virology, 67 (1). pp. 507-515. ISSN 0022-538X

URL: http://www.ncbi.nlm.nih.gov/pubmed/8093222

Abstract

The adenovirus E1A 243R oncoprotein stimulates expression from the promoter of the human proliferating cell nuclear antigen (PCNA). To gain insight into the mechanism of activation, we analyzed deletion and point mutations of the 243R protein for their abilities to activate PCNA promoter-directed reporter gene expression upon cotransfection into HeLa cells. Large deletions that in combination span the entire protein severely impaired the ability of EIA 243R to induce PCNA expression. Smaller deletions and specific point mutations that target specific E1A-binding proteins were less deleterious to PCNA induction. The data suggest that E1A activates transcription of the PCNA gene by multiple mechanisms and that, of the known 243R-associated proteins, p300 and p107-cyclin A can mediate the response while p105-RB does not appear to participate. Presumably, the functional redundancy ensures that 243R can activate expression of this essential DNA replication protein regardless of cell type and physiological conditions.

Item Type: Paper
Uncontrolled Keywords: RETINOBLASTOMA GENE-PRODUCT E2F TRANSCRIPTION FACTOR PAPILLOMAVIRUS TYPE-16 E7 DNA POLYMERASE-DELTA HUMAN CYCLIN-A E1A PROTEINS 19-KILODALTON PROTEIN MOLECULAR-CLONING AUXILIARY PROTEIN MESSENGER-RNAS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Proliferating Cell Nuclear Antigen
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: January 1993
Date Deposited: 14 Apr 2016 18:33
Last Modified: 14 Apr 2016 18:33
PMCID: PMC237388
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32554

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