Vimentin Expression Is Differentially Regulated by Il-2 and Il-4 in Murine T-Cells

Hornbeck, P. V., Garrels, J. I., Capetanaki, Y., Heimer, S. (October 1993) Vimentin Expression Is Differentially Regulated by Il-2 and Il-4 in Murine T-Cells. Journal of Immunology, 151 (8). pp. 4013-4021. ISSN 0022-1767

URL: http://www.ncbi.nlm.nih.gov/pubmed/7691937

Abstract

IL-2 and IL-4 are T cell growth factors that are produced by different T cell subsets and have distinct roles in lymphocyte biology. Despite their importance in the immune system, little is known about the genes that these lymphokines may specifically control and the interaction of these lymphokines in regulating the expression of their target genes. In this paper, we use the factor-dependent murine T cell line (CT.4R) to investigate the interaction of IL-2 and IL-4 in regulating gene expression. We report that the intermediate filament protein vimentin is differentially regulated by these lymphokines. Cells grown in IL-2 typically express 10- to 20-fold more vimentin and vimentin RNA than those grown in IL-4, but express similar levels of other cytoskeletal proteins including actin and tubulin. Vimentin was specifically induced by IL-2 and apparently suppressed by IL-4 in normal lymph node T cells, suggesting that its differential regulation by these lymphokines is physiologically relevant. We investigated the synergy between IL-2 and IL-4 in regulating the expression of vimentin RNA and compared it to that of two other lymphokine-responsive genes, pancreatic lipase and the IL-2Ralpha subunit. Complex regulatory interactions were revealed: IL-4 suppressed the ability of IL-2 to induce vimentin RNA but not IL-2Ralpha RNA, whereas IL-2 inhibited the ability of IL-4 to induce lipase RNA. These results indicate that IL-2 and IL-4 can cross-regulate lymphokine-responsive genes and can simultaneously exert both positive and negative regulation of different genes within the same cell.

Item Type: Paper
Uncontrolled Keywords: MOUSE LYMPHOCYTES-T PROTEIN KINASE-C INTERMEDIATE FILAMENTS IL-2-DEPENDENT PROLIFERATION PROMOTES GROWTH GENE-EXPRESSION CROSS-LINKING INTERLEUKIN-2 RECEPTOR ACTIVATION
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > immune cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > immune cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > immune cell
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 15 October 1993
Date Deposited: 14 Apr 2016 15:56
Last Modified: 14 Apr 2016 15:56
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32549

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