The VP16 accessory protein HCF is a family of polypeptides processed from a large precursor protein

Wilson, A. C., LaMarco, K., Peterson, M. G., Herr, W. (July 1993) The VP16 accessory protein HCF is a family of polypeptides processed from a large precursor protein. Cell, 74 (1). pp. 115-25. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8392914
DOI: 10.1016/0092-8674(93)90299-6

Abstract

Upon lytic infection of permissive cells, the herpes simplex virus (HSV) transactivator protein VP16 associates with an accessory protein termed host cell factor (HCF). Binding to HCF activates VP16 for association with the octamer motif-binding protein Oct-1, to form a multiprotein-DNA complex responsible for activating transcription of the HSV immediate early genes. We show that HCF comprises a series of related polypeptides that range from 110 to 300 kd, all of which are encoded by a single gene. Although there is no obvious sequence similarity between HCF and other known proteins, HCF contains eight repeats of a new 26 amino acid motif. cDNAs encoding HCF predict a large open reading frame of 2035 codons. When expressed in human cells, this large open reading frame encodes both the 300 kd and smaller HCF polypeptides, indicating that the smaller polypeptides arise by processing of the 300 kd protein.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence DNA-Binding Proteins/metabolism Herpes Simplex Virus Protein Vmw65/ genetics/metabolism Host Cell Factor C1 Humans Molecular Sequence Data Octamer Transcription Factor-1 Open Reading Frames Protein Binding Protein Precursors/ metabolism Protein Processing, Post-Translational Repetitive Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Simplexvirus Transcription Factors/ genetics/metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > herpes simplex virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: 16 July 1993
Date Deposited: 21 Apr 2016 15:06
Last Modified: 21 Apr 2016 15:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32496

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving