Telomeres Shorten during Aging of Human Fibroblasts

Harley, C. B., Futcher, A. B., Greider, C. W. (1990) Telomeres Shorten during Aging of Human Fibroblasts. Nature, 345 (6274). pp. 458-460. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/2342578
DOI: 10.1038/345458a0

Abstract

The terminus of a DNA helix has been called its Achilles' heel. Thus to prevent possible incomplete replication and instability of the termini of linear DNA, eukaryotic chromosomes end in characteristic repetitive DNA sequences within specialized structures called telomeres. In immortal cells, loss of telomeric DNA due to degradation or incomplete replication is apparently balanced by telomere elongation, which may involve de novo synthesis of additional repeats by novel DNA polymerase called telomerase. Such a polymerase has been recently detected in HeLa cells. It has been proposed that the finite doubling capacity of normal mammalian cells is due to a loss of telomeric DNA and eventual deletion of essential sequences. In yeast, the est1 mutation causes gradual loss of telomeric DNA and eventual cell death mimicking senescence in higher eukaryotic cells. Here, we show that the amount and length of telomeric DNA in human fibroblasts does in fact decrease as a function of serial passage during ageing in vitro and possibly in vivo. It is not known whether this loss of DNA has a causal role in senescence.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres
CSHL Authors:
Communities: CSHL labs > Futcher lab
CSHL labs > Greider lab
Depositing User: Matt Covey
Date Deposited: 09 Feb 2016 17:43
Last Modified: 09 Feb 2016 17:43
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32345

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