Tat-responsive region RNA of human immunodeficiency virus 1 can prevent activation of the double-stranded-RNA-activated protein kinase

Gunnery, S., Rice, A. P., Robertson, H. D., Mathews, M. B. (November 1990) Tat-responsive region RNA of human immunodeficiency virus 1 can prevent activation of the double-stranded-RNA-activated protein kinase. Proc Natl Acad Sci U S A, 87 (22). pp. 8687-91. ISSN 0027-8424 (Print)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2247437
DOI: 10.1073/pnas.87.22.8687

Abstract

Transcription from the human immunodeficiency virus type 1 promoter gives rise to short cytoplasmic transcripts of approximately 60 nucleotides as well as to longer mRNAs. These RNAs contain the Tat-responsive region sequence, which is capable of assuming a stem-loop structure and has been implicated in the regulation of both transcription and translation. It has been reported that Tat-responsive region RNA inhibits translation in vitro through activation of an interferon-induced protein kinase, the double-stranded-RNA-activated inhibitor, which phosphorylates eukaryotic initiation factor 2. We show that the activation property is due to double-stranded RNA that often contaminates RNA synthesized in vitro using bacteriophage RNA polymerases. After purification, high concentrations of Tat-responsive region RNA inhibit the activation of double-stranded RNA-activated inhibitor, suggesting that it may serve to protect human immunodeficiency virus type 1 infection from a cellular defense mechanism.

Item Type: Paper
Uncontrolled Keywords: Cloning, Molecular Enzyme Activation Gene Products, tat/physiology *HIV Long Terminal Repeat HIV-1/*genetics In Vitro Techniques Protein Kinases/*metabolism RNA, Double-Stranded/genetics/isolation & purification RNA, Viral/genetics/isolation & purification *Regulatory Sequences, Nucleic Acid Ribonucleases/pharmacology eIF-2 Kinase tat Gene Products, Human Immunodeficiency Virus
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: November 1990
Date Deposited: 09 Feb 2016 16:59
Last Modified: 09 Nov 2017 20:38
PMCID: PMC55024
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32343

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