Gunnery, S., Rice, A. P., Robertson, H. D., Mathews, M. B. (November 1990) Tat-responsive region RNA of human immunodeficiency virus 1 can prevent activation of the double-stranded-RNA-activated protein kinase. Proc Natl Acad Sci U S A, 87 (22). pp. 8687-91. ISSN 0027-8424 (Print)0027-8424 (Linking)
Preview |
PDF (Paper)
Mathews PNAS 1990.pdf - Published Version Download (1MB) | Preview |
Abstract
Transcription from the human immunodeficiency virus type 1 promoter gives rise to short cytoplasmic transcripts of approximately 60 nucleotides as well as to longer mRNAs. These RNAs contain the Tat-responsive region sequence, which is capable of assuming a stem-loop structure and has been implicated in the regulation of both transcription and translation. It has been reported that Tat-responsive region RNA inhibits translation in vitro through activation of an interferon-induced protein kinase, the double-stranded-RNA-activated inhibitor, which phosphorylates eukaryotic initiation factor 2. We show that the activation property is due to double-stranded RNA that often contaminates RNA synthesized in vitro using bacteriophage RNA polymerases. After purification, high concentrations of Tat-responsive region RNA inhibit the activation of double-stranded RNA-activated inhibitor, suggesting that it may serve to protect human immunodeficiency virus type 1 infection from a cellular defense mechanism.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cloning, Molecular Enzyme Activation Gene Products, tat/physiology *HIV Long Terminal Repeat HIV-1/*genetics In Vitro Techniques Protein Kinases/*metabolism RNA, Double-Stranded/genetics/isolation & purification RNA, Viral/genetics/isolation & purification *Regulatory Sequences, Nucleic Acid Ribonucleases/pharmacology eIF-2 Kinase tat Gene Products, Human Immunodeficiency Virus |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | November 1990 |
| Date Deposited: | 09 Feb 2016 16:59 |
| Last Modified: | 09 Nov 2017 20:38 |
| PMCID: | PMC55024 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32343 |
Actions (login required)
 |
Administrator's edit/view item |