Ccd-Based Detector for Protein Crystallography with Synchrotron X-Rays

Strauss, M. G., Westbrook, E. M., Naday, I., Coleman, T. A., Westbrook, M. L., Travis, D. J., Sweet, R. M., Pflugrath, J. W., Stanton, M. (1990) Ccd-Based Detector for Protein Crystallography with Synchrotron X-Rays. Nuclear Instruments & Methods in Physics Research Section a-Accelerators Spectrometers Detectors and Associated Equipment, 297 (1-2). pp. 275-295. ISSN 0168-9002

DOI: 10.1016/0168-9002(90)91376-M


A detector with a 114 mm aperture, based on a charge-coupled device (CCD), has been designed for X-ray diffraction studies in protein crystallography. The detector was tested at the National Synchrotron Light Source with a beam intensity, through a 0.3 mm collimator, of greater than 10(9) X-ray photons/s. A fiberoptic taper, an image intensifier, and a lens demagnify, intensify, and focus the image onto a CCD having 512 x 512 pixels. The statistical uncertainty in the detector output was evaluated as a function of conversion gain. From this, a detective quantum efficiency (DQE) of 0.36 was derived. The dynamic range of a 4 x 4 pixel resolution element, comparable in size to a diffraction peak, was 10(4). The point-spread function shows FWHM resolution of approximately 1 pixel, where a pixel is 160-mu-m on the detector face. A data set collected from a chicken egg-white lysozyme crystal, consisting of 495 0.1-degrees frames, was processed by the MADNES data reduction program. The symmetry R-factors for the data were 3.2-3.5%. In a separate experiment a complete lysozyme data set consisting of 45 1-degrees frames was obtained in just 36 s of X-ray exposure. Diffraction images from crystals of the myosin S1 head (a = 275 angstrom) were also recorded; the Bragg spots, only 5 pixels apart, were separated but not fully resolved. Changes in the detector design that will improve the DQE and spatial resolution are outlined. The overall performance showed that this type of detector is well suited for X-ray scattering investigations with synchrotron sources.

Item Type: Paper
Uncontrolled Keywords: area detectors diffraction radiation sensor
Subjects: Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 05 Apr 2016 19:48
Last Modified: 05 Apr 2016 19:48

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