The equine major plasma serpin multigene family: partial characterization including sequence of the reactive-site regions

Patterson, S. D., Bell, K., Shaw, D. C. (October 1991) The equine major plasma serpin multigene family: partial characterization including sequence of the reactive-site regions. Biochem Genet, 29 (9-10). pp. 477-99. ISSN 0006-2928 (Print)0006-2928 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1772402
DOI: 10.1007/BF02399689

Abstract

The equine Pi system, which is highly polymorphic and was considered to be controlled by a single locus, has been shown to be controlled by four loci (named Spi 1-4). This system is the equine equivalent of the major human plasma serpin (serine protease inhibitor), human alpha 1 PI. Twenty-two haplotypes of the equine Pi system have been characterized by two-dimensional electrophoresis, resulting in the assignment of pI, Mr, and bovine trypsin and chymotrypsin inhibition characteristics to 109 proteins. These proteins have been analyzed further to determine their relatedness to each other as well as to human alpha 1 PI using immunochemical, structural, and functional criteria. The amino acid sequences of the N termini and reactive-site regions have been determined on proteins from each of the four equine Spi loci. This allowed the designation of the proteins from the Spi 1 locus as being METserpins and the functional equivalents of human alpha 1 PI. The Spi 4 proteins are ARGserpins, and by alignment the Spi 2 proteins are ILEserpins, the first so far described. The P1 residue for the Spi 3 proteins was unable to be determined. The limited peptide and immunopeptide mapping revealed that proteins from all four loci were closely related, but within the four there were two pairs (Spi 1 and 2 and Spi 3 and 4) which were more related. All were probably derived from the same gene that gave rise to human alpha 1 PI.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Binding Sites Haplotypes Horses/*genetics Molecular Sequence Data *Multigene Family N-Acetylneuraminic Acid Oxidation-Reduction Peptide Mapping Polymorphism, Genetic Serpins/blood/chemistry/*genetics/metabolism Sialic Acids/analysis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease > serine protease inhibitor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease > serpin
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: October 1991
Date Deposited: 11 Jan 2016 16:10
Last Modified: 11 Jan 2016 16:10
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32072

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving