Role of the apical stem in maintaining the structure and function of adenovirus virus-associated RNA

Mellits, K. H., Pe'ery, T., Mathews, M. B. (April 1992) Role of the apical stem in maintaining the structure and function of adenovirus virus-associated RNA. J Virol, 66 (4). pp. 2369-77. ISSN 0022-538X (Print)0022-538X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1548768

Abstract

Adenovirus virus-associated (VA) RNAI maintains efficient protein synthesis during the late phase of infection by preventing the activation of the double-stranded-RNA-dependent protein kinase, DAI. A secondary structure model for VA RNAI predicts the existence of two stems joined by a complex stem-loop structure, the central domain. The structural consequences of mutations and compensating mutations introduced into the apical stem lend support to this model. In transient expression assays for VA RNA function, foreign sequences inserted into the apical stem were fully tolerated provided that the stem remained intact. Mutants in which the base of the apical stem was disrupted retained partial activity, but truncation of the apical stem abolished the ability of the RNA to block DAI activation in vitro, suggesting that the length and position of the stem are both important for VA RNA function. These results imply that VA RNAI activity depends on secondary structure at the top of the apical stem as well as in the central domain and are consistent with a two-step mechanism involving DAI interactions with both the apical stem and the central domain.

Item Type: Paper
Uncontrolled Keywords: Adenoviruses, Human/*genetics/physiology Base Sequence Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation RNA, Viral/*chemistry/physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
organism description > virus > adeno associated virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: April 1992
Date Deposited: 28 Sep 2015 15:02
Last Modified: 28 Sep 2015 15:02
PMCID: PMC289032
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31811

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