Dual modes of control of c-fos mRNA induction by intracellular calcium in T cells

Lee, G., Gilman, M. (July 1994) Dual modes of control of c-fos mRNA induction by intracellular calcium in T cells. Mol Cell Biol, 14 (7). pp. 4579-87. ISSN 0270-7306 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8007962
DOI: 10.1128/MCB.14.7.4579

Abstract

Cytoplasmic calcium is a nearly universal second messenger in eukaryotes. In many cell types, elevated intracellular calcium interacts synergistically with inducers of protein kinase C to elicit activation of complete biological programs normally induced by extracellular signals. In T cells, elevated cytoplasmic calcium is a critical mediator of activation in response to stimulation of the antigen receptor, and in some T-cell lines, treatment with a combination of calcium ionophore and protein kinase C activator mimics authentic antigen treatment. The synergistic interaction of calcium and protein kinase C in T cells is also observed at the level of gene expression. Here we examine the molecular mechanisms through which these agents exert synergistic control over the expression of the c-fos proto-oncogene in a T-cell hybridoma. We find that the principal effect of calcium is on the elongation of c-fos transcripts. This step constitutes the major control of c-fos mRNA accumulation in these cells. In addition, calcium regulates the initiation of c-fos transcription. This effect requires the serum response element of the c-fos gene and an additional sequence immediately 3' to this element. Thus, calcium regulates c-fos expression through at least two distinct molecular pathways.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Calcium/ metabolism Cell Line Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase/biosynthesis Columbidae Cytoplasm/metabolism Gene Expression/drug effects Gene Expression Regulation/drug effects Genes, fos Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides Promoter Regions (Genetics) Protein Kinase C/metabolism Proto-Oncogene Proteins c-fos/ biosynthesis RNA, Messenger/ biosynthesis Research Support, U.S. Gov't, P.H.S. Second Messenger Systems Signal Transduction T-Lymphocytes/drug effects/ metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic/drug effects/physiology Transfection
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells

organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > calcium channel
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: July 1994
Date Deposited: 09 Jul 2015 18:56
Last Modified: 09 Jul 2015 18:56
PMCID: PMC358830
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31436

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