Clonal dynamics of native haematopoiesis

Sun, J., Ramos, A., Chapman, B., Johnnidis, J. B., Le, L., Ho, Y. J., Klein, A., Hofmann, O., Camargo, F. D. (October 2014) Clonal dynamics of native haematopoiesis. Nature, 514. pp. 322-327. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/25296256
DOI: 10.1038/nature13824

Abstract

It is currently thought that life-long blood cell production is driven by the action of a small number of multipotent haematopoietic stem cells. Evidence supporting this view has been largely acquired through the use of functional assays involving transplantation. However, whether these mechanisms also govern native non-transplant haematopoiesis is entirely unclear. Here we have established a novel experimental model in mice where cells can be uniquely and genetically labelled in situ to address this question. Using this approach, we have performed longitudinal analyses of clonal dynamics in adult mice that reveal unprecedented features of native haematopoiesis. In contrast to what occurs following transplantation, steady-state blood production is maintained by the successive recruitment of thousands of clones, each with a minimal contribution to mature progeny. Our results demonstrate that a large number of long-lived progenitors, rather than classically defined haematopoietic stem cells, are the main drivers of steady-state haematopoiesis during most of adulthood. Our results also have implications for understanding the cellular origin of haematopoietic disease.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:
Communities: CSHL labs > Hammell M. lab
Watson School > Publications
Depositing User: Matt Covey
Date: 5 October 2014
Date Deposited: 20 Oct 2014 15:05
Last Modified: 19 Dec 2014 14:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30843

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