Identification of a novel Rac1-interacting protein involved in membrane ruffling

Van Aelst, L., Joneson, T., Bar-Sagi, D. (August 1996) Identification of a novel Rac1-interacting protein involved in membrane ruffling. Embo Journal, 15 (15). pp. 3778-86. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/8670882

Abstract

The Rac GTP binding proteins are implicated in actin cytoskeleton-membrane interaction in mammalian cells. In fibroblast cells, Rac has been shown to mediate growth factor-induced polymerization of actin to form membrane ruffles and lamellipodia. We report here the isolation of a noval Rac1-interacting protein, POR1. POR1 binds directly to Rac1, and the interaction of POR1 with Rac1 is GTP dependent. A mutation in the Rac1 effector binding loop shown to abolish membrane ruffling also abolishes interaction with POR1. Truncated versions of POR1 inhibit the induction of membrane ruffling by an activated mutant of Rac1, V12Rac1, in quiescent rat embryonic fibroblast REF52 cells. Furthermore, POR1 synergizes with an activated mutant of Ras, V12Ras, in the induction of membrane ruffling. These results suggest a potential role for POR1 in Rac1-mediated signaling pathways.

Item Type: Paper
Uncontrolled Keywords: Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Base Sequence Blotting, Northern Carrier Proteins/genetics/ isolation & purification/metabolism Cell Membrane/metabolism Evolution, Molecular GTP-Binding Proteins/ metabolism Guanosine Triphosphate/metabolism Humans Molecular Sequence Data Point Mutation Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Signal Transduction rac GTP-Binding Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Rac
evolution
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTP binding protein
organs, tissues, organelles, cell types and functions > tissues types and functions > membranes
organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
Depositing User: Kathleen Darby
Date: 1 August 1996
Date Deposited: 12 May 2014 16:29
Last Modified: 12 May 2014 16:29
PMCID: PMC452058
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30120

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