N-Oct 5 is generated by in vitro proteolysis of the neural POU-domain protein N-Oct 3

Atanasoski, S., Schreiber, E., Fontana, A., Herr, W. (March 1997) N-Oct 5 is generated by in vitro proteolysis of the neural POU-domain protein N-Oct 3. Oncogene, 14 (11). pp. 1287-94. ISSN 0950-9232 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9178889

Abstract

The neural POU-domain proteins N-Oct 3 and N-Oct 5 were first identified in electrophoretic mobility retardation assays through their ability to bind to the octamer sequence ATGCAAAT. These two N-Oct factors are detected in extracts from tumor-derived and normal neural cells. They are present differentially, however, in extracts from melanocytes and melanoma cells: N-Oct 3 is present in extracts from both melanocytes and melanoma cells, whereas N-Oct 5 is more evident in extracts from metastatic melanoma cells. We show here that a cDNA encoding N-Oct 3 directs synthesis of both the N-Oct 3 and N-Oct 5 proteins and that the N-Oct 5 protein in neural and melanoma-cell extracts is also related to N-Oct 3. N-Oct 5, however, is apparently not expressed in vivo: It is not detected if cells are rapidly lysed in SDS or if extracts are prepared with a cocktail of protease inhibitors that includes the serine-protease inhibitor 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF). These data suggest that N-Oct 5 is a specific in vitro proteolytic cleavage product of N-Oct 3 and is not directly related to melanocyte malignancy.

Item Type: Paper
Uncontrolled Keywords: Animals Astrocytes/metabolism COS Cells Cell Transformation, Neoplastic/genetics DNA, Complementary Homeodomain Proteins Humans Hydrolysis Melanoma/genetics/pathology POU Domain Factors Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription Factors/genetics/ metabolism Tumor Cells, Cultured
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein
diseases & disorders > cancer > cancer types > melanomas
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Kathleen Darby
Date: 20 March 1997
Date Deposited: 08 May 2014 16:01
Last Modified: 08 May 2014 16:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29991

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