NMDA receptor activity stabilizes presynaptic retinotectal axons and postsynaptic optic tectal cell dendrites in vivo

Rajan, I., Witte, S., Cline, H. T. (February 1999) NMDA receptor activity stabilizes presynaptic retinotectal axons and postsynaptic optic tectal cell dendrites in vivo. Journal of neurobiology, 38 (3). pp. 357-68. ISSN 0022-3034 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10022578
DOI: 10.1002/(SICI)1097-4695(19990215)38:3<357::AID-NEU5>3.0.CO;2-#

Abstract

To investigate the role of N-methyl-D-aspartate (NMDA) receptor activity in the stability of the presynaptic axon arbor and postsynaptic dendritic arbors in vivo, we took time-lapse confocal images of single DiI-labeled Xenopus retinotectal axons and optic tectal neurons in the presence and absence of the NMDA receptor antagonist, APV. Retinotectal axons or tectal neurons were imaged at 30-min intervals over 2 h, or twice over a 24-h period. Retinal axons in animals exposed to DL-APV (100 microM) showed an increase in rates of branch additions and a decrease in branch lifetimes over 2 h compared to untreated axons. Under the same experimental conditions, tectal neurons showed a decreased rate of branch tip additions and retractions. APV treatment over 24 h had no apparent effect on axon arbor morphology, but did decrease tectal cell dendritic arbor elaboration. These observations demonstrate that NMDA receptor activity in postsynaptic neurons stabilizes pre- and postsynaptic neuronal morphology in vivo.. However, when NMDA receptor activity is blocked, presynaptic retinal axons respond with increased arbor dynamics while postsynaptic tectal cell dendrites decrease arbor dynamics. Such differential responses of pre- and postsynaptic partners might increase the probability of coactive afferents converging onto a common target under conditions of lower NMDA receptor activity.

Item Type: Paper
Uncontrolled Keywords: Animals Axons/ physiology Brain Mapping Dendrites/ physiology Image Processing, Computer-Assisted Microscopy, Confocal Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/ physiology Receptors, Presynaptic/ physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Retina/cytology/ physiology Superior Colliculus/cytology/ physiology Xenopus laevis
Subjects: Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
organs, tissues, organelles, cell types and functions > tissues types and functions > axon
Investigative techniques and equipment > microscopy
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Kathleen Darby
Date: 15 February 1999
Date Deposited: 30 Apr 2014 15:59
Last Modified: 07 May 2014 12:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29793

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