HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions

Neuwald, A. F., Hirano, T. (2000) HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions. Genome Research, 10 (10). pp. 1445-1452. ISSN 1088-9051

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11042144
DOI: 10.1101/gr.147400

Abstract

HEAT repeats correspond to tandemly arranged curlicue-like structures that appear to serve as flexible scaffolding on which other components can assemble. Using sensitive sequence analysis techniques we detected HEAT repeals in various chromosome-associated proteins, including four Families of proteins associated with condensins and cohesins, which are nuclear complexes that contain structural maintenance of chromosome (SMC) proteins. Among the proteins detected were the XCAP-D2 and XCAP-G subunits of the Xenopus laevis 13S condensin complex, the Aspergillus BimD and Sordaria macrospora Spo76p proteins, the budding yeast Scc2p protein, and the related Drosophila transcriptional activator Nipped-B. Clathrin adaptor and COP-I coatomer subunits, which Function in vesicle coat assembly and were previously noted to share weak sequence similarity to condensin subunits, also contain HEAT repeats. HEAT repeats were also found in the TBP-associated TIP120 protein, a global enhancer of transcription, and in the budding yeast Motlp protein, which is a member of the SWI2/SNF2 family. SWI2/SNF2 proteins, some of which are helicases, perform diverse roles in transcription control, DNA repair, and chromosome segregation and form chromatin-remodeling complexes. HEAT repeats also were found in disl-TOG family and cofactor D family microtubule-associated proteins, which, owing to their roles in microtubule dynamics, perform functions related to mitotic progression and chromosome segregation. Hence, our analysis predicts structural Features of these proteins and suggests that HEAT repeats may play important roles in chromosome dynamics.

Item Type: Paper
Uncontrolled Keywords: CYANOBACTERIAL PHYCOBILISOMES PROTEIN COMPLEXES SCAFFOLD PROTEIN IMPORTIN-ALPHA FISSION YEAST IN-VITRO SMC CONDENSATION DROSOPHILA ACTIVATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cohesin complex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > condensin complex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Hirano lab
CSHL labs > Neuwald lab
Depositing User: Matt Covey
Date Deposited: 31 Jan 2014 14:39
Last Modified: 31 Jan 2014 14:39
PMCID: PMC310966
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29397

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