Functional overlap of sequences that activate transcription and signal ubiquitin-mediated proteolysis

Salghetti, S. E., Muratani, M., Wijnen, H., Futcher, B., Tansey, W. P. (March 2000) Functional overlap of sequences that activate transcription and signal ubiquitin-mediated proteolysis. Proceedings of the National Academy of Sciences of the United States of America, 97 (7). pp. 3118-3123. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/10706616
DOI: 10.1073/pnas.97.7.3118

Abstract

Many transcription factors, particularly those involved in the control of cell growth, are unstable proteins destroyed by ubiquitin-mediated proteolysis. In a previous study of sequences targeting the transcription factor Myc for destruction, we observed that the region in Myc signaling ubiquitin-mediated proteolysis overlaps closely with the region in Myc that activates transcription. Here, we present evidence that the overlap of these two activities is not unique to Myc, but reflects a more general phenomenon. We show that a similar overlap of activation domains and destruction elements occurs in other unstable transcription factors and report a close correlation between the ability of an acidic activation domain to activate transcription and to signal proteolysis. We also show that destruction elements from yeast cyclins, when tethered to a DNA-binding domain, activate transcription. The intimate overlap of activation domains and destruction elements reveals an unexpected convergence of two very different processes and suggests that transcription factors may he destroyed because of their ability to activate transcription.

Item Type: Paper
Uncontrolled Keywords: INDUCIBLE FACTOR 1-ALPHA RNA-POLYMERASE-II SACCHAROMYCES-CEREVISIAE 26S PROTEASOME C-JUN CYCLIN DEGRADATION GENE-PRODUCT BETA-CATENIN DNA-BINDING G1 CYCLINS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein ubiquitination
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Futcher lab
CSHL labs > Tansey lab
Watson School > Publications
Depositing User: Matt Covey
Date: March 2000
Date Deposited: 30 Jan 2014 21:56
Last Modified: 26 Sep 2014 15:14
PMCID: PMC16202
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29391

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