Crystal structure of the functional domain of the splicing factor Prp18

Jiang, J. Z., Horowitz, D. S., Xu, R. M. (March 2000) Crystal structure of the functional domain of the splicing factor Prp18. Proceedings of the National Academy of Sciences of the United States of America, 97 (7). pp. 3022-3027. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/10737784
DOI: 10.1073/pnas.97.7.3022

Abstract

The splicing factor Prp18 is required for the second step of pre-mRNA splicing, We have isolated and determined the crystal structure of a large fragment of the Saccharomyces cerevisiae Prp18 that lacks the N-terminal 79 amino acids. This fragment called Prp18 Delta 79. is fully active in yeast splicing in vitro and includes the sequences of Prp18 that have been evolutionarily conserved, The core structure of Prp18 Delta 79 is compact and globular, consisting of five a-helices that adopt a novel fold that we have designated the five-helix X-bundle. The structure suggests that one face of Prp18 interacts with the splicing factor Slu7, whereas the more evolutionarily conserved amino acids in Prp18 form the opposite face, The most highly conserved region of Prp18, a nearly invariant stretch of 19 aa, forms part of a loop between two alpha-helices and may interact with the Us small nuclear ribonucleoprotein particles, The structure is consistent with a model in which Prp18 forms a bridge between Slu7 and the U5 small nuclear ribonucleoprotein particles.

Item Type: Paper
Uncontrolled Keywords: 2ND CATALYTIC STEP SACCHAROMYCES-CEREVISIAE RNA YEAST PROTEIN SLU7 SPLICEOSOME SITES CRYSTALLOGRAPHY 3'-SPLICE-SITE
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Xu lab
Depositing User: Matt Covey
Date: March 2000
Date Deposited: 31 Jan 2014 14:58
Last Modified: 31 Jan 2014 14:58
PMCID: PMC16185
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29370

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