A positioned nucleosome on the human U6 promoter allows recruitment of SNAP(c) by the Oct-1 POU domain

Zhao, X. Y., Pendergrast, P. S., Hernandez, N. (March 2001) A positioned nucleosome on the human U6 promoter allows recruitment of SNAP(c) by the Oct-1 POU domain. Molecular Cell, 7 (3). pp. 539-549. ISSN 1097-2765

URL: http://www.ncbi.nlm.nih.gov/pubmed/11463379
DOI: 10.1016/S1097-2765(01)00201-5

Abstract

The human snRNA promoters contain a proximal sequence element (PSE) required for basal transcription and a distal sequence element (DSE) required for activated transcription. The PSE recruits the multisubunit factor SNAP,, whereas the DSE recruits Oct-1. Oct-1 and SNAP, bind cooperatively to DNA when their respective binding sites are moved into proximity through a mechanism that involves a defined protein-protein contact. Here, we show that on the natural U6 promoter, cooperative binding of Oct-1 and SNAP, is mediated by a positioned nucleosome that resides between the DSE and the PSE. This cooperative binding requires the same protein-protein contact as cooperative binding to closely spaced sites on naked DNA and mediates transcription activation.

Item Type: Paper
Uncontrolled Keywords: RNA-POLYMERASE-II SMALL NUCLEAR-RNA DNA-BINDING DOMAIN ACTIVATES TRANSCRIPTION GENE-TRANSCRIPTION COMPLEX SNAP(C) DISTAL PROMOTER SNRNA PROMOTERS CHROMATIN REGION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nucleosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Matt Covey
Date: March 2001
Date Deposited: 15 Jan 2014 21:13
Last Modified: 15 Jan 2014 21:13
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29335

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