Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex

Chong, S. S., Hu, P., Hernandez, N. (June 2001) Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex. Journal of Biological Chemistry, 276 (23). pp. 20727-20734. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/11279001
DOI: 10.1074/jbc.M100088200

Abstract

The human U6 small nuclear (sn) RNA core promoter consists of a proximal sequence element, which recruits the multisubunit factor SNAP(c), and a TATA box, which recruits the TATA box-binding protein, TBP, In addition to SNAP(c) and TBP, transcription from the human U6 promoter requires two well defined factors. The first is hB", a human homologue of the B" subunit of yeast TFIIIB generally required for transcription of RNA polymerase III genes, and the second is hBRFU, one of two human homologues of the yeast TFIIIB subunit BRF specifically required for transcription of U6-type RNA polymerase III promoters. Here, we have partially puri fled and characterized a RNA polymerase III complex that can direct transcription from the human U6 promoter when combined with recombinant SNAP(c), recombinant TBP, recombinant hB", and recombinant hBRFU, These results open the way to reconstitution of U6 transcription from entirely defined components.

Item Type: Paper
Uncontrolled Keywords: PROXIMAL SEQUENCE ELEMENT SACCHAROMYCES-CEREVISIAE ENCODES 2 FUNCTIONAL COMPONENTS TERMINATION FACTOR LA BINDING PROTEIN LA HUMAN SNRNA GENES C III INVITRO TRANSCRIPTION SUBUNIT COMMON HUMAN HOMOLOG
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Matt Covey
Date: June 2001
Date Deposited: 22 Jan 2014 19:56
Last Modified: 22 Jan 2014 19:56
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29224

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