Nitric oxide triggers a switch to growth arrest during differentiation of neuronal cells

Peunova, N., Enikolopov, G. (1995) Nitric oxide triggers a switch to growth arrest during differentiation of neuronal cells. Nature, 375 (6526). pp. 68-73. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/7536899
DOI: 10.1038/375068a0

Abstract

ARREST Of cell division is a prerequisite for cells to enter a program of terminal differentiation. Mitogenesis and cytostasis of neuronal cell precursors can be induced by the same or by different growth or trophic factors(1-9). Response of PC12 cells to nerve growth factor (NGF) involves a proliferative phase that is followed by growth arrest and differentiation. Here we present evidence that the cytostatic effect of NGF is mediated by nitric oxide (NO), a second messenger molecule with both para- and autocrine properties ties that can diffuse freely and act within a restricted volume(10-14). We show that NGF induces different forms of nitric oxide synthase (NOS) in neuronal cells, that nitric oxide (NO) acts as a cytostatic agent in these cells, that inhibition of NOS leads to reversal of NGF-induced cytostasis and thereby prevents full differentiation, and that capacity of a mutant cell line to differentiate can be rescued by exogenous NO, We suggest that induction of NOS is an important step in the commitment of neuronal precursors and that NOS serves as a growth arrest gene, initiating the switch to cytostasis during differentiation.

Item Type: Paper
Uncontrolled Keywords: CENTRAL-NERVOUS-SYSTEM SMOOTH-MUSCLE CELLS RIBONUCLEOTIDE REDUCTASE NADPH DIAPHORASE SYNTHASE PROLIFERATION CLONING MACROPHAGES HEPATOCYTES GENE
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > small molecules > nitric oxide
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date Deposited: 20 Dec 2013 16:05
Last Modified: 20 Dec 2013 16:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29141

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