Mutual regulation between the intercellular messengers nitric oxide and brain-derived neurotrophic factor in rodent neocortical neurons

Xiong, H. B., Yamada, K., Han, D. K., Nabeshima, T., Enikolopov, G., Carnahan, J., Nawa, H. (1999) Mutual regulation between the intercellular messengers nitric oxide and brain-derived neurotrophic factor in rodent neocortical neurons. European Journal of Neuroscience, 11 (5). pp. 1567-1576. ISSN 0953-816X

URL: http://www.ncbi.nlm.nih.gov/pubmed/10215909
DOI: 10.1046/j.1460-9568.1999.00567.x

Abstract

The diffusible factors, nitric oxide (NO) and brain-derived neurotrophic factor (BDNF) are both suggested to be intercellular messengers that have similar synaptic activities and developmental influences in the brain. In the present study, we have analysed their mutual regulation with respect to their production in rodent neocortical neurons. Some of the cultured rat neocortical neurons exhibited immunoreactivity for both neuronal NO synthase (NOS) and the BDNF receptor trkB. Neuronal NOS appeared to be activated autonomously and produced NO in culture as monitored by nitrite accumulation. Inhibition of the endogenous NO production in culture by a NOS inhibitor, N-G-monomethyl-L-arginine (NMMA), enhanced basal expression of BDNF mRNA and protein. Similarly, cerebroventricular administration of another NOS inhibitor, N-omega-nitro-L-arginine methylester (L-NAME), but not D-NAME or saline, increased BDNF content in the neocortex. In the opposite direction, however, BDNF appeared to function as a positive regulator for NO synthesis. Addition of BDNF upregulated the neuronal NOS expression as well as NO production in neocortical culture. In agreement, BDNF knock-out mice exhibited significant impairment of neuronal NOS expression in the neocortex. Taken together, these observations suggest that the trans-synaptic signalling molecules, NO and BDNF, influence the production of each other and mutually regulate the strength of their intercellular communications.

Item Type: Paper
Uncontrolled Keywords: knock-out mouse neurotrophin nitrite trkB LONG-TERM POTENTIATION ADULT-RAT FOREBRAIN SYNAPTIC TRANSMISSION POSTSYNAPTIC DENSITY L-ARGININE IN-VIVO NEUROPEPTIDE EXPRESSION ANTEROGRADE TRANSPORT HIPPOCAMPAL-NEURONS MOLECULAR-CLONING
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > brain derived neurotrophic factor
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > small molecules > nitric oxide
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date Deposited: 20 Dec 2013 15:49
Last Modified: 20 Dec 2013 15:49
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29137

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