Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs

Krainer, A. R. (October 1988) Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs. Nucleic Acids Research, 16 (20). pp. 9415-9429. ISSN 0305-1048

URL: http://www.ncbi.nlm.nih.gov/pubmed/3141901
DOI: 10.1093/nar/16.20.9415

Abstract

The four major nucleoplasmic small nuclear ribonucleoprotein particles U1, U2, U4/U6 and U5 can be extensively purified from HeLa cells by immunoaffinity chromatography using a monoclonal anti-trimethylguanosine antibody. The snRNP particles in active splicing extracts are selectively bound to the immunoaffinity matrix, and are then gently eluted by competition with an excess of free nucleoside. Biochemical complementation studies show that the purified snRNPs are active in pre-mRNA splicing, but only in the presence of additional non-snRNP protein factors. All the RNPs that are necessary for splicing can be purified in this manner. The active snRNPs are characterized with respect to their polypeptide composition, and shown to be distinct from several other activities implicated in splicing.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: October 1988
Date Deposited: 10 Dec 2013 21:21
Last Modified: 10 Dec 2013 21:21
PMCID: PMC338753
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28964

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