Functions of SR proteins in the U12-dependent AT-AC pre-mRNA splicing pathway

Hastings, M. L., Krainer, A. R. (March 2001) Functions of SR proteins in the U12-dependent AT-AC pre-mRNA splicing pathway. RNA, 7 (3). pp. 471-482. ISSN 1355-8382

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11333026
DOI: 10.1017/s1355838201002552

Abstract

SR proteins play critical roles in the major pre-mRNA splicing pathway. A second pathway processes U12-dependent AT-AC introns, We demonstrate, by biochemical complementation, the requirement for SR proteins in splicing of AT-AC introns. Whereas SR proteins were sufficient to activate splicing of a P120 AT-AC intron, splicing of a sodium channel AT-AC intron required an additional nuclear fraction. Individual recombinant SR proteins promoted splicing of both substrates, but displayed marked preferences. SR proteins supported basal AT-AC splicing, and also splicing stimulation via a downstream enhancer or conventional 5 ' splice site. Analysis of chimeric transcripts revealed that information dispersed throughout exons and introns dictates SR protein specificity and the requirement for the additional nuclear fraction. Thus, SR proteins function in both major and minor splicing pathways, and in coordinating the activities of both spliceosomes via exon definition. These results suggest that despite the substantial differences in intron consensus sequences and in four of the five snRNPs in each spliceosome, at least some of the interactions involving SR proteins are conserved between the two pathways.

Item Type: Paper
Uncontrolled Keywords: AT-AC introns exon definition exonic splicing enhancer pre-mRNA splicing SR proteins factor u2af(35) site selection introns requires premessenger rna exon definition u1 snrnp messenger nuclear recognition identification
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: March 2001
Date Deposited: 11 Dec 2013 19:41
Last Modified: 11 Dec 2013 19:43
PMCID: PMC1370102
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28911

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