Soluble Guanylate Cyclase Generation of cGMP Regulates Migration of MGE Neurons

Mandal, S., Stanco, A., Buys, E. S., Enikolopov, G., Rubenstein, J. L. (2013) Soluble Guanylate Cyclase Generation of cGMP Regulates Migration of MGE Neurons. Journal of Neuroscience, 33 (43). pp. 16897-914. ISSN 1529-2401 (Electronic)0270-6474 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/24155296
DOI: 10.1523/jneurosci.1871-13.2013

Abstract

Here we have provided evidence that nitric oxide-cyclic GMP (NO-cGMP) signaling regulates neurite length and migration of immature neurons derived from the medial ganglionic eminence (MGE). Dlx1/2(-/-) and Lhx6(-/-) mouse mutants, which exhibit MGE interneuron migration defects, have reduced expression of the gene encoding the alpha subunit of a soluble guanylate cyclase (Gucy1A3). Furthermore, Dlx1/2(-/-) mouse mutants have reduced expression of NO synthase 1 (NOS1). Gucy1A3(-/-) mice have a transient reduction in cortical interneuron number. Pharmacological inhibition of soluble guanylate cyclase and NOS activity rapidly induces neurite retraction of MGE cells in vitro and in slice culture and robustly inhibits cell migration from the MGE and caudal ganglionic eminence. We provide evidence that these cellular phenotypes are mediated by activation of the Rho signaling pathway and inhibition of myosin light chain phosphatase activity.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > nitric oxide synthase
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Microscopy Service
Depositing User: Matt Covey
Date Deposited: 05 Nov 2013 21:08
Last Modified: 26 Dec 2014 15:12
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28844

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