Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting

Eide, T., Carlson, C., Tasken, K. A., Hirano, T., Tasken, K., Collas, P. (May 2002) Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting. Embo Reports, 3 (5). pp. 426-432. ISSN 1469-221X

URL: http://www.ncbi.nlm.nih.gov/pubmed/11964380
DOI: 10.1093/embo-reports/kvf089

Abstract

A-kinase (or PKA)-anchoring protein AKAP95 is a zinc-finger protein implicated in mitotic chromosome condensation by acting as a targeting molecule for the condensin complex. We have identified determinants of chromatin-binding, condensin-targeting and chromosome-condensation activities of AKAP95. Binding of AKAP95 to chromatin is conferred by residues 387–450 and requires zinc finger ZF1. Residues 525–569 are essential for condensation of AKAP95-free chromatin and condensin recruitment to chromosomes. Mutation of either zinc finger of AKAP95 abolishes condensation. However, ZF1 is dispensable for condensin targeting, whereas the C-terminal ZF2 is required. AKAP95 interacts with Xenopus XCAP-H condensin subunit in vitro and in vivo but not with the human hCAP-D2 subunit. The data illustrate the involvement of overlapping, but distinct, domains of AKAP95 for condensin recruitment and chromosome condensation and argue for a key role of ZF1 in chromosome condensation and ZF2 in condensin targeting. Moreover, condensin recruitment to chromatin is not sufficient to promote condensation.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > zinc finger proteins
CSHL Authors:
Communities: CSHL labs > Hirano lab
Depositing User: Matt Covey
Date: May 2002
Date Deposited: 08 Jan 2014 20:41
Last Modified: 08 Jan 2014 20:41
PMCID: PMC1084102
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28699

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