Gr1+ myeloid cells are a patrolling subpopulation of the tumor-infiltrating myeloid cells.

Egeblad, M., Ewald, A. J., Askautrud, H. A., Truitt, M. L., Welm, B. E., Bainbridge, E., Peeters, G., Krummel, M. F., Werb, Z. (2008) Gr1+ myeloid cells are a patrolling subpopulation of the tumor-infiltrating myeloid cells. [Video]

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Abstract

Gr1+ myeloid cells are a patrolling subpopulation of the tumor-infiltrating myeloid cells. Gr1+/c-fms+ myeloid cells (red/green) patrol in tumor vasculature (yellow) in the tumor-stroma border area while c-fms+/dextran+ macrophages (green/yellow) do not migrate in the same area. Gr1+ cells were labeled with Alexa-Fluor-647-conjugated anti-Gr1 antibodies (red), which were coinjected, i.v., with 70 kD rhodamine-conjugated dextran (yellow) in an MMTV-PyMT;ACTB-ECFP;c-fms-EGFP mouse.Dextran first labeled the blood vessels and was taken up by macrophages over time. Shown are the ECFP (blue, mostly epithelial cells), EGFP (green, myeloid cells), rhodamine (yellow, dextran) and Alexa-Fluor-647 (red, Gr1+ antibodies) channels. The movie is shown first with and then without the dextran channel. Time indicates time after injection of antibody and dextran. From Egeblad et al. (2008) Dis. Model. Mech., 1 155-167.

Item Type: Video
Subjects: diseases & disorders > cancer
diseases & disorders
Investigative techniques and equipment
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
Investigative techniques and equipment > microscopy
CSHL Authors:
Communities: CSHL labs > Egeblad lab
Depositing User: Matt Covey
Date: 2008
Date Deposited: 26 Apr 2013 16:07
Last Modified: 26 Apr 2013 16:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28262

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