An adipose tissue-specific β-adrenergic receptor. Molecular cloning and down-regulation in obesity

Muzzin, P., Revelli, J. P., Kuhne, F., Gocayne, J. D., McCombie, W. R., Venter, J. C., Giacobino, J. P., Fraser, C. M. (1991) An adipose tissue-specific β-adrenergic receptor. Molecular cloning and down-regulation in obesity. Journal of Biological Chemistry, 266 (35). pp. 24053-24058. ISSN 00219258 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1721063

Abstract

Clones encoding an atypical β-adrenergic receptor were isolated from a rat brown adipose tissue cDNA library. This receptor expressed in Chinese hamster ovary (CHO) cells displays a low affinity for β-adrenergic antagonists and a high affinity for BRL 37344, an agonist that selectively stimulates lipolysis in adipose tissue. The rank order of potency for agonist-mediated increases in intracellular cAMP in transfected cells correlates with that for agonist-mediated stimulation of lipolysis in brown adipocytes. Northern blot analysis demonstrates that this receptor subtype is expressed only in brown and white adipose tissue where it represents the predominant β-receptor subtype. The amount of atypical β-adrenergic receptor present in adipose tissue of obese (fa/fa) Zucker rats is reduced by up to 71% as compared with lean (Fa/Fa) control animals. These findings suggest that a change in the expression of this β-adrenergic receptor subtype may play a role in obesity.

Item Type: Paper
Uncontrolled Keywords: beta adrenergic receptor adipose tissue animal model animal tissue article dna sequence down regulation molecular cloning nonhuman obesity priority journal rat Adrenergic beta-Agonists Adrenergic beta-Antagonists Amino Acid Sequence Animal Base Sequence Brown Fat CHO Cells Cloning, Molecular Comparative Study DNA Down-Regulation Gene Library Hamsters Human Kinetics Male Molecular Sequence Data Poly A Radioligand Assay Rats Rats, Inbred Strains Receptors, Adrenergic, beta RNA RNA, Messenger Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 20:04
Last Modified: 25 Apr 2013 20:04
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28191

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving