Muzzin, P., Revelli, J. P., Kuhne, F., Gocayne, J. D., McCombie, W. R., Venter, J. C., Giacobino, J. P., Fraser, C. M. (1991) An adipose tissue-specific β-adrenergic receptor. Molecular cloning and down-regulation in obesity. Journal of Biological Chemistry, 266 (35). pp. 24053-24058. ISSN 00219258 (ISSN)
Abstract
Clones encoding an atypical β-adrenergic receptor were isolated from a rat brown adipose tissue cDNA library. This receptor expressed in Chinese hamster ovary (CHO) cells displays a low affinity for β-adrenergic antagonists and a high affinity for BRL 37344, an agonist that selectively stimulates lipolysis in adipose tissue. The rank order of potency for agonist-mediated increases in intracellular cAMP in transfected cells correlates with that for agonist-mediated stimulation of lipolysis in brown adipocytes. Northern blot analysis demonstrates that this receptor subtype is expressed only in brown and white adipose tissue where it represents the predominant β-receptor subtype. The amount of atypical β-adrenergic receptor present in adipose tissue of obese (fa/fa) Zucker rats is reduced by up to 71% as compared with lean (Fa/Fa) control animals. These findings suggest that a change in the expression of this β-adrenergic receptor subtype may play a role in obesity.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | beta adrenergic receptor adipose tissue animal model animal tissue article dna sequence down regulation molecular cloning nonhuman obesity priority journal rat Adrenergic beta-Agonists Adrenergic beta-Antagonists Amino Acid Sequence Animal Base Sequence Brown Fat CHO Cells Cloning, Molecular Comparative Study DNA Down-Regulation Gene Library Hamsters Human Kinetics Male Molecular Sequence Data Poly A Radioligand Assay Rats Rats, Inbred Strains Receptors, Adrenergic, beta RNA RNA, Messenger Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Transfection |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab |
| Depositing User: | Matt Covey |
| Date: | 1991 |
| Date Deposited: | 25 Apr 2013 20:04 |
| Last Modified: | 25 Apr 2013 20:04 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28191 |
Actions (login required)
 |
Administrator's edit/view item |