Mitotic Functions for SNAP45, a Subunit of the Small Nuclear RNA-activating Protein Complex SNAPc

Shanmugam, M., Hernandez, N. (May 2008) Mitotic Functions for SNAP45, a Subunit of the Small Nuclear RNA-activating Protein Complex SNAPc. J Biol Chem, 283 (21). pp. 14845-56.

URL: http://www.ncbi.nlm.nih.gov/pubmed/18356157
DOI: 10.1074/jbc.M800833200

Abstract

The small nuclear RNA-activating protein complex SNAP(c) is required for transcription of small nuclear RNA genes and binds to a proximal sequence element in their promoters. SNAP(c) contains five types of subunits stably associated with each other. Here we show that one of these polypeptides, SNAP45, also known as PTF delta, localizes to centrosomes during parts of mitosis, as well as to the spindle midzone during anaphase and the mid-body during telophase. Consistent with localization to these mitotic structures, both down- and up-regulation of SNAP45 lead to a G(2)/M arrest with cells displaying abnormal mitotic structures. In contrast, down-regulation of SNAP190, another SNAP(c) subunit, leads to an accumulation of cells with a G(0)/G(1) DNA content. These results are consistent with the proposal that SNAP45 plays two roles in the cell, one as a subunit of the transcription factor SNAP(c) and another as a factor required for proper mitotic progression.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SNAP protein
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle
organs, tissues, organelles, cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Matt Covey
Date: 23 May 2008
Date Deposited: 26 Feb 2013 16:08
Last Modified: 26 Feb 2013 16:08
PMCID: PMC2386947
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27572

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