Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies

Campbell, R. A. A., Honegger, K. S., Gruntman, E., Turner, G. C. (2012) Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies. In: Genetically Encoded Functional Indicators. Neuromethods, 72 . Humana Press, pp. 113-124. ISBN 978-1-62703-013-7

URL: http://link.springer.com/protocol/10.1007%2F978-1-...
DOI: 10.1007/978-1-62703-014-4_7

Abstract

Genetically encoded calcium indicators make it possible to track neural activity on a population-wide level. Here we describe a preparation that enables two-photon imaging of neural activity in an essentially intact fly. We present strategies to minimize motion of the brain, both in preparation technique and in apparatus design. We discuss key variables for reducing the problems of photobleaching and phototoxicity in order to collect high quality imaging data. Finally, we discuss approaches to analyze the large quantities of data that can now be readily acquired using the latest generation of genetically encoded calcium indicators.

Item Type: Book Section
Uncontrolled Keywords: Calcium imaging Two-photon microscopy GCaMP Mushroom body Drosophila Olfaction Memory
Subjects: organism description > animal > insect > Drosophila
Investigative techniques and equipment
Investigative techniques and equipment > microscopy > flourescence microscopy
organism description > animal > insect
Investigative techniques and equipment > microscopy
Investigative techniques and equipment > microscopy > flourescence microscopy > two-photon excitation microscopy
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Turner lab
Watson School > Publications
Depositing User: Matt Covey
Date: 2012
Date Deposited: 31 Jan 2013 21:10
Last Modified: 03 Jan 2019 19:17
URI: http://repository.cshl.edu/id/eprint/26916

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