Temporally regulated nuclear entry of the Drosophila period protein contributes to the circadian clock

Curtin, K. D., Huang, Z. J., Rosbash, M. (1995) Temporally regulated nuclear entry of the Drosophila period protein contributes to the circadian clock. Neuron, 14 (2). pp. 365-372. ISSN 08966273 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7857645
DOI: 10.1016/0896-6273(95)90292-9

Abstract

The Drosophila period protein (PER) is a predominantly nuclear protein and a likely component of a circadian clock. PER is required for daily oscillations in the transcription of its own gene and thus participates in a circadian feedback loop. In this study, key pacemaker neurons of the Drosophila brain were examined to determine whether the subcellular distribution of PER changes with the time of day. Indeed, PER was found to accumulate in the cytoplasm for several hours before entering the nucleus during a narrow time window. Three long-period mutations (per(L)) cause a delay in the timing of nuclear translocation and a further delay at elevated temperature. The data indicate that regulation of PER nuclear entry is critical for circadian oscillations by providing a necessary temporal delay between PER synthesis and its effect on transcription.

Item Type: Paper
Uncontrolled Keywords: nuclear protein animal cell article circadian rhythm controlled study drosophila genetic transcription intracellular transport nonhuman priority journal protein synthesis protein transport transcription regulation Animal Cell Nucleus Feedback Gene Expression Regulation Immunohistochemistry Kinetics Motor Activity Mutagenesis Nuclear Proteins Subcellular Fractions Temperature Time Factors Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs > Huang lab
Depositing User: CSHL Librarian
Date Deposited: 11 Apr 2012 16:16
Last Modified: 10 May 2013 19:18
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25791

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving