Temporal gene regulation during HIV-1 infection of human CD4+ T cells

Corbeil, J., Sheeter, D., Genini, D., Rought, S., Leoni, L., Du, P., Ferguson, M., Masys, D. R., Welsh, J. B., Fink, J. L., Sasik, R., Huang, D., Drenkow, J., Richman, D. D., Gingeras, T. R. (2001) Temporal gene regulation during HIV-1 infection of human CD4+ T cells. Genome Research, 11 (7). pp. 1198-1204. ISSN 10889051 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11435401
DOI: 10.1101/gr.GR-1802R

Abstract

CD4+ T-cell depletion is a characteristic of human immunodeficiency virus type 1 (HIV-1) infection. In this study, modulation of mRNA expression of 6800 genes was monitored simultaneously at eight time points in a CD4+ T-cell line (CEM-GFP) during HIV infection. The responses to infection included: (1) >30% decrease at 72 h after infection in overall host-cell production of monitored mRNA synthesis, with the replacement of host-cell mRNA by viral mRNA, (2) suppression of the expression of selected mitochondrial and DNA repair gene transcripts, (3) increased expression of the proapoptotic gene and its gene p53-induced product Bax, and (4) activation of caspases 2, 3, and 9. The intense HIV-1 transcription resulted in the repression of much cellular RNA expression and was associated with the induction of apoptosis of infected cells but not bystander cells. This choreographed host gene response indicated that the subversion of the cell transcriptional machinery for the purpose of HIV-1 replication is skin to genotoxic stress and represents a major factor leading to HIV-induced apoptosis.

Item Type: Paper
Uncontrolled Keywords: caspase 2 caspase 3 caspase 9 CD4 antigen messenger RNA protein Bax protein p53 apoptosis cell line controlled study DNA repair gene control gene expression gene repression genetic transcription genotoxicity host cell human human cell Human immunodeficiency virus 1 Human immunodeficiency virus infection letter mitochondrion monitoring priority journal RNA synthesis T lymphocyte virus replication CD4-Positive T-Lymphocytes Cell Line Transformed G2 Phase Gene Expression Regulation, Viral Green Fluorescent Proteins HIV-1 Humans Luminescent Proteins Lymphocyte Count Mitosis Transcription, Genetic Virion Eukaryota Human immunodeficiency virus RNA viruses
Subjects: diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 2001
Date Deposited: 13 Mar 2012 15:07
Last Modified: 17 Jul 2013 20:51
PMCID: PMC311116
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25279

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